Biochemical characterization of the purple form of Marinobacter hydrocarbonoclasticus nitrous oxide reductase

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Biochemical characterization of the purple form of Marinobacter hydrocarbonoclasticus nitrous oxide reductase.

Nitrous oxide reductase (N(2)OR) catalyses the final step of the denitrification pathway-the reduction of nitrous oxide to nitrogen. The catalytic centre (CuZ) is a unique tetranuclear copper centre bridged by inorganic sulphur in a tetrahedron arrangement that can have different oxidation states. Previously, Marinobacter hydrocarbonoclasticus N(2)OR was isolated with the CuZ centre as CuZ*, in...

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After growth in the absence of nitrogenous oxides under anaerobic phototrophic conditions, several strains of Rhodopseudomonas capsulata were shown to possess a nitrous oxide reductase activity. The enzyme responsible for this activity had a periplasmic location and resembled a nitrous oxide reductase purified from Pseudomonas perfectomarinus. Electron flow to nitrous oxide reductase was couple...

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ژورنال

عنوان ژورنال: Philosophical Transactions of the Royal Society B: Biological Sciences

سال: 2012

ISSN: 0962-8436,1471-2970

DOI: 10.1098/rstb.2011.0311